Neurological disorders are often associated with neuronal dysfunction. Neuroblastoma (NB) cell line SH-SY5Y is widely used as in vitro model as it can differentiate into neuron-like cells. Many other NB cell lines can also respond to differentiation treatment but their potential in neuronal modeling remained unexplored. We evaluated 18 NB cell lines using RNA-seq and differentiation treatment with retinoic acid and brain-derived neurotrophic factor. Only few mutations in neurotransmitter pathway