Cynodon dactylon, a tropical grass has been traditionally used in folk medicine to treat various ailments. The present study aimed to extract the root part of Cynodon dactylon and evaluate the anticoagulant activity. The extract was prepared by using maceration method with aqueous & non aqueous solvent and evaluation of it’s anticoagulant activity by using blood clotting time measurement method. The extract was subjected to preliminary photochemical screening which gives the confirmation of pres