BACKGROUND Dendrobium officinale (D. officinale ) is a traditional Chinese herb that has been studied extensively for its medicinal properties, including gastrointestinal protection, anti-aging effects, and antioxidant properties. However, the molecular mechanisms by which D. officinale delays aging have not been fully elucidated. AIM To investigate the effects of D. officinale extract (DOE) on extending healthy lifespan through the maintenance of intestinal homeostasis, and to explore its inter