Timed batch inputs unlock substantially higher yields for enzymatic cascades

Nature Chemistry, Published online: 06 May 2026; doi:10.1038/s41557-026-02138-1 Optimizing the performance of complex enzymatic reaction networks remains challenging as hidden interactions and unfavourable kinetic barriers often arise when enzymes operate together in networks. Now a model-guided design strategy for generating time-dependent protocols for batch reactions has been developed. This strategy offers a general approach for optimizing multi-step reaction sequences.