Benchmarking of shotgun sequencing depth reveals the potential and limitations of shallow metagenomics and strain-level analysis
Thomas Clavel
Nature Microbiology, Published online: 21 April 2026; doi:10.1038/s41564-026-02334-2 A comparison of sequencing of complex mock communities performed at multiple depths and analysed using different methods establishes minimum standards that can ensure the accuracy of metagenomic analysis for a particular objective.