Tourism inevitably induces ecological impacts on soil in tourist destinations while contributing to regional economic development. Previous studies have well documented the negative effects of trampling on soil physical structure and nutrient dynamics; however, the consequences for soil hydrolytic enzyme activities and their potential vegetation-dependent differences remain poorly understood. Here, we examined trampling-induced changes in soil hydrolytic enzyme activities in planted grasslands, secondary deciduous forests, and secondary coniferous forests on Jigong Mountain, Central China. Our results showed that: (1) planted grasslands had significantly lower baseline soil hydrolytic enzyme contents compared to the secondary forests; (2) tourist trampling reduced soil hydrolytic enzyme contents by 11.7-53.0% across three vegetation types, with planted grasslands exhibiting less reduction (11.7-40.5%) than coniferous (25.0-53.0%) and deciduous forests (32.6-50.9%), indicating grassland soil had a stronger resistance to trampling than the other two types; (3) multiple stepwise regression analysis revealed that decreased soil total nitrogen by trampling was the predominant regulator of soil hydrolytic enzyme contents across different vegetation types. Our findings suggest that trampling homogenizes soil hydrolytic enzyme activity among different vegetation types, indicating a loss of spatial heterogeneity in microbial-driven biochemical processes. The finding that soils from different vegetation types showed different response magnitudes to trampling indicates that the design of tourist routes can be adjusted based on such differences to reduce the negative impacts caused by trampling.