Mycoplasma bovis is a significant bacterial pathogen involved in the Bovine Respiratory Disease (BRD) complex. While assessing localized mucosal immunity is vital for understanding host defenses against respiratory pathogens, there is a lack of validated diagnostic assays for detecting mucosal immunoglobulins. This study aimed to evaluate the diagnostic performance of a novel multiplex ELISA platform (Pictor PictVet®) in detecting anti-M. bovis IgG in bovine nasopharyngeal swab washes. Archived