IntroductionPhosphorus (P) is a critical nutrient influencing soil fertility, yet its role in shaping bacterial community structure remains underexplored, particularly in native grasslands versus agricultural systems. This study aimed to evaluate how bioavailable P and other edaphic factors influence soil bacterial communities across contrasting land-use types.MethodsSoil bacterial communities were assessed using 16S rRNA gene-based amplicon sequencing at two contrasting sites in northeast Kansa