Integration of an iterative factor into a validated HPTLC method for quantification of koumine in the toxic plant Gelsemium elegans Benth
Mun Fei Yam
BackgroundGelsemium elegans (Gardner and Champ.) Benth. (Gelsemiaceae), commonly known as heartbreak grass, is a popular herb to treat a variety of ailments, native to China and Southeast Asia. Its toxicity is attributed to the indole alkaloid it contains, particularly koumine.MethodsIn this research, the iterative factor was integrated into a validated high-performance thin-layer chromatography (HPTLC) method to enhance the precision and reliability of koumine quantification. To establish the v