Biochemical and Biophysical Research Communications

Objective To investigate the role of miR-31-5p in non-small cell lung cancer (NSCLC) cells and its function in an in vitro blood-brain barrier (BBB) model, and to elucidate the underlying regulatory mechanisms. Methods RT-qPCR was used to determine the relative expression levels of miR-31-5p, QKI mRNA, and TUG1 in serum samples obtained from the healthy control group and patients with NSCLC brain…

The cyclin-dependent kinase inhibitor p16 Ink4a is a widely used marker of cellular senescence; however, the contribution of p16 Ink4a -expressing hepatocytes to hepatic steatosis in metabolic dysfunction-associated steatohepatitis (MASH) remains unclear. Here, we combined p16-CreER-tdTomato lineage tracing, single-cell transcriptomic analysis, and intersectional hepatocyte-specific ablation usin…

Nephronectin (Npnt) is a multifunctional basement membrane protein containing EGF-like repeats and an RGD motif. While we previously reported that Npnt regulates dental epithelial stem cell proliferation via its EGF-like repeats, the specific role of its RGD motif in ameloblast differentiation remained to be elucidated. In this study, we investigated the domain-specific functions of Npnt and iden…