Analytical and Bioanalytical Chemistry

Abstract Untargeted urinary metabolomics represents a promising approach for investigating metabolic alterations associated with oncogenic processes such as breast cancer (BC). However, the stable selection of informative m/z features remains a central challenge in biomarker-oriented studies, particularly in the context of early BC detection and population-level screening. Urine samples from two …

Abstract The evolution of metabolomics, a field aimed at comprehensively measuring the small organic molecule composition of a biological matrix, fundamentally reshaped the landscape of analytical measurement reliability. The 2011 release of Standard Reference Material (SRM) 1950 Metabolites in Frozen Human Plasma by the National Institute of Standards and Technology (NIST) marked the first refer…

Abstract Light activation is a promising approach for lowering the power consumption and increasing the selectivity of chemoresistive gas sensors based on semiconducting metal oxides. In this study, different aspects of commonly used materials are investigated, compared, and evaluated for their application as gas sensors. Their ability to detect CO and NO $$_{2}$$ 2 at low temperature (70 $$^{\ci…

Plant-based milk alternatives (PBMAs) are expanding rapidly, motivating the development of fast, minimal-prep analytical tools for their analysis. We collected and analyzed ( n = 154) commercial PBMAs (almond, oat, rice, soy, coconut) from Austrian and Croatian supermarkets using attenuated total reflection mid‑infrared (ATR‑MIR) and diffuse reflection near‑infrared (DR‑NIR) spectroscopy to compa…

This study presents the development of an AChE@ZIF-8 composite biosensor using a gold nanoparticle (AuNPs) probe as a naked-eye signal readout platform for the sensitive detection of carbamate pesticides. As a biomarker for carbaryl monitoring, we present the encapsulation of acetylcholinesterase within zeolitic imidazolate frameworks-8 (AChE@ZIF-8) to enhance its stability without losing its int…

Most MS-based workflows for N-terminal protein sequence analysis rely on the modification of primary amines at the protein N-terminus and lysine residues, in many cases through dimethylation. However, incomplete dimethyl labeling and undesired side reactions have been reported previously and were also observed in our experiments. In this study, we evaluated alternative methods for N-terminal prot…