ABSTRACT This study demonstrates that circadian rhythm disruption induced by night‐shift work is associated with elevated blood pressure in both murine models and human subjects. By integrating rodent and human epidemiological data, we identify mitochondrial dysfunction as the principal mechanistic contributor to this relationship. In mice, data were obtained at 4 and 10 weeks, with sampling performed at both ZT0 and ZT12. Simulated night shift work elevates blood pressure, induces circadian gen