Objectives: Streptococcus pneumoniae is a major respiratory pathogen. Reports during the COVID-19 era suggest decreased pneumococcal activity. This study aimed to assess the frequency of S. pneumoniae in adult sputum cultures and its antimicrobial susceptibility in a tertiary care hospital. Materials and Methods: This retrospective laboratory-based study was conducted in a tertiary care hospital in Turkey. Sputum cultures from adult patients between October 1, 2021, and October 1, 2023, were ana