Extracellular vesicles (EVs) are lipid bilayer-enclosed nanoparticles secreted by all cells. They can be released by producing cells to dispose of unwanted material but can also convey signals or transfer biomolecules between cells. Therefore, EVs are currently considered as potential delivery vehicles for therapeutic purposes. EV delivery of cargo into the cytosol of recipient cells typically involves sequential steps: binding to the cell surface, internalization, and endosomal escape leading t