Background The formation of secondary cell walls (SCWs) determines lignocellulosic composition and cell wall architecture. NAC transcription factors ( NAM / ATAF1 / CUC2 ) act as master regulators of the SCW transcriptional network. This study characterized MfSWN1 , a Miscanthus floridulus NAC transcription factor homologous to NAC regulators of SCW synthesis, using Arabidopsis thaliana as a heterologous system. Methods The wild-type (Col-0), the SCW-deficient double mutant nst1–1 nst3-1 , MfSWN
