Efficient Secretory Expression of Soybean Peroxidase in Saccharomyces cerevisiae Through Systematic Engineering

Soybean peroxidase (SBP), a thermostable and pH-robust enzyme, holds promise as a biopolymer cross-linking agent. This study pioneers the secretory expression of SBP in <i>Saccharomyces cerevisiae</i>, a GRAS (generally recognized as safe) microbial host. To address expression bottlenecks, a synthetic hybrid promoter was designed through modular assembly, enabling stringent glucose-dependent repression and high induction ratios. Transcriptional efficiency was further optimized via systematic ter