Elucidating HLTF-Mediated DNA Fork Remodeling via Native Mass Spectrometry

Replication fork reversal (RFR) is a crucial DNA damage tolerance mechanism that protects genome stability by remodeling DNA fork structures. The helicase-like transcription factor (HLTF) is one of the key components in the RFR process and is responsible for the conversion of a stalled replication fork into a four-way reversed fork, mediated by its ATPase activity. In contrast to a wealth of biochemical evidence depicting the biological activities of HLTF, very little information is available on