Developing a Predation-Oriented Metabolomics Pipeline to Uncover New Antibiotics from Myxobacteria

The threat of antimicrobial resistance demands the exploration of underexplored microbial resources and innovative discovery methodologies. We introduce a strategy integrating predation-oriented strain prioritization with NMR-based metabolomics to accelerate antibiotic discovery from myxobacteria. By combining ecological prioritization, chromatographic prefractionation, universal NMR fingerprinting, and chemometric deconvolution, we achieved rapid dereplication of known metabolites and selective