Local Nixtamalization Processes Reduce Aflatoxin B1 Concentration and Shift Metabolome in Naturally Contaminated Maize

Nixtamalization is an alkaline cooking process known to lower aflatoxin B1 (AFB1) in maize, yet reported efficacy varies (28-100% reduction). Thus, existing data are not sufficient to inform nixtamalization practices in specific local contexts. To investigate this, we conducted a controlled lab study using AFB1-contaminated Guatemalan maize to evaluate the effect of current rural Guatemalan cooking practices on the AFB1 concentration. We evaluated nine conditions varying calcium hydroxide concen