A hydrophobic core in the coiled‐coil domain is essential for NRC resistosome function
Hung‐Yu Wang·Chueh‐Hung Wu·Yifeng Chen·Bing‐Jen Chiang·Cristina Vuolo·S. F. Jenkins·Freddie J. King·Kim‐Teng Lee·Foong‐Jing Goh·Tarhan Ibrahim·Tolga O. Bozkurt·Enoch Lok Him Yuen
The nucleotide-binding leucine-rich repeat protein (NLR) required for cell death (NRC) family represents a group of helper NLRs that are required by sensor NLRs to execute hypersensitive cell death during pathogen infection. NRCs contain an N-terminal coiled-coil (CC) domain essential for their function, yet our knowledge of how this domain contributes to NRC function remains limited. Using site-directed mutagenesis and transient expression in Nicotiana benthamiana, we screened conserved hydroph