Optimization of Juncao Substrate Formulation for Flammulina filiformis Cultivation: An Enzymatic and Transcriptomic Study
Weizhen Huang·Dongmei Lin·Haitao Chen·Jiali Lai·Ben Menda Ukii·Lin Zhang·Yaojin Wang·Yuan Luo·Zhanxi Lin·Junru Wang
Flammulina filiformis is a significant edible and medicinal fungus; however, its industrial expansion has been limited by traditional cultivation practices, highlighting an urgent need for resource-efficient and environmentally friendly alternative substrates. This study investigated the partial replacement of traditional substrates with Cenchrus fungigraminus. Utilizing the simplex-lattice method for optimization, we identified an optimal cultivation formulation, composed primarily of 20% C. fu