Temporal dynamics of protein phosphorylation and S ‐nitrosylation direct maize seed germination programs
Wenyang Zhang·Shijuan Yan·W. Y. Li·Yan Wei·Shaowen Wu·Danting Zhuo·M Ye·Yuanyuan Chen·Wenjie Huang·Yong Li·Kainan Xu·Jianbin Lai·Alisdair R. Fernie·Yanjiao Wang
SUMMARY Seed germination directly influences seedling establishment and ultimately crop yield. While hormonal and transcriptional regulations of germination are well characterized, the role of post‐translational modifications (PTMs) remains largely unexplored. In this resource article, we developed a phosphate‐affinity tag‐switch (PAT‐switch) proteomics workflow enabling simultaneous, site‐specific profiling of phosphorylation and S ‐nitrosylation during maize seed germination. Using this strate