Analysis of Nanosensor-Reported Waveforms for Plant Wounding

Using nanosensors in living plants allows the real-time detection of internal reactive oxygen species (e.g., hydrogen peroxide) signaling in response to environmental stressors. The time-dependent pulse of hydrogen peroxide (H₂O₂) constitutes a signaling waveform; however, standardized methods of extracting and analyzing such waveforms quantitatively remain elusive. Here, we develop a reference-less framework to extract stress-induced H₂O₂ wavef