Caffeic Acid–Chicoric Acid (1:1) Mixture Exerts Antioxidant Effects and Regulates Intestinal Health in Oxazolone-Induced Zebrafish

Caffeic acid (CaA) and chicoric acid (ChA) each exhibit significant antioxidant activity when used alone, yet their combined effects on antioxidant capacity and intestinal health in zebrafish remain unclear. This study used isobolographic analysis to identify a 1:1 ratio of CaA to ChA as optimal for synergistic antioxidant activity, with its ABTS and DPPH IC50 (21.65 μg/mL, 69.66 μg/mL) outperforming single monomers in vitro. In an oxazolone (Oxa)-induced zebrafish intestinal oxidative stress mo