Serological surveillance of avian influenza virus H9N2 subtype in occupational populations exposed to poultry environment in China during 2018–2023

Abstract Background Avian influenza virus (AIV) H9N2 have a major role in the emergence of influenza pandemic. We assessed the risk of AIV H9N2 to the human population and public health. Methods The hemagglutination inhibition method was used to screen for hemagglutinin antibodies. Microneutralization tests were performed to confirm neutralizing antibodies against the AIV H9N2 subtype. Real-time PCR was conducted to detect the H9 subtype in environmental samples. GraphPad Prism software was used