Extracellular vesicles (EVs) are fundamental intercellular signaling organelles; these lipid-bilayer-enclosed particles are secreted by all cell types and carry a cargo of proteins, lipids, and nucleic acids to facilitate such cell communication and transfer. Advances in the field of single EV analyses, including profiling techniques, capture and detection strategies, digital technologies, multiplexing capability, machine learning, and resolution of molecular analysis have led new insights into