Suppression of pre-B cell colony formation by catecholamine oxidation

The sympathetic nervous system regulates many aspects of lymphocyte function by stimulating adrenergic receptors with catecholamines. To determine the effects of β-adrenergic receptor (β-AR) signaling on B cell development, we utilized the pre-B cell colony forming unit (CFU-Pre-B) assay and found that isoproterenol (ISO), a β-AR selective catecholamine, potently suppresses pre-B cell colony formation. Further investigation using selective agonists and knockout mice showed that the colony suppre