Extraction of bioactive phenolic compounds from yerba mate leaves using ultrasound: HPLC profiling and statistical analysis of variables

Extraction is critical step in extracting bioactive phytochemicals from plant sources and separating them. In this study, a Box-Benken (BBD) design was used, with 15 experiments conducted to optimize the ultrasonic extraction process. Three independent variables (ethanol concentration, temperature, and time) were examined to assess their effect on total extract yield (TYE) as the main response. The optimization aimed to maximize the extraction of active compounds from the resilient matrix of yer