Docking-Assisted Hybridization Chain Reaction Strategy Based on Bifunctional Hairpin Probes for Ultrasensitive Analysis of 17β-Estradiol

17β-Estradiol (E2), an endogenous estrogen, can be present in milk and may accumulate during processing, potentially contributing to long-term dietary exposure that raises concerns for consumer health. Regulatory agencies have established intake limits to minimize risk, highlighting the importance of reliable monitoring. In this study, we developed a docking-guided dual-mode aptasensor for sensitive, selective, and practical detection of E2 in complex dairy matrices. Molecular docking analyses r