Sustainable chromatographic assays of a novel antifungal combination for keratomycosis

Keratomycosis, a sight-threatening fungal infection of the cornea, can be more effectively treated with a novel combination of Natamycin (NAT, 5%) and Fluconazole (FLC, 0.2%) than with NAT alone. To support the development of this therapy, new sustainable, eco-friendly, and sensitive analytical methods were developed for the simultaneous quantification of both drugs in pharmaceutical formulations. Two techniques were successfully employed. The first was a Reversed-Phase High-Performance Liquid C