The present study was designed to evaluate the anti-inflammatory and immunomodulatory potential of the ethanolic extract of Cucumis sativus peel (EECS) using in vitro and in vivo experimental models. The extract was obtained as a greenish-brown solid with a percentage yield of 21.5%. Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, terpenoids, tannins, saponins, and phenolic compounds. Quantitative estimation demonstrated a high total phenolic content (161.05 ±