Integrated Purification Process for 5-Aminolevulinic Acid Phosphate Produced via Biological Fermentation

5-aminolevulinic acid (5-ALA) affords various positive health effects, including benefits for conditions such as diabetes. Biological fermentation holds potential for efficiently mass-producing biomolecules, including 5-ALA, yet it generally results in a mixture of target molecules and impurities, including byproducts. Pyrazine-2,5-dipropionic acid (PY), a dimer of 5-ALA, can easily form in 5-ALA production and is one of its major impurities. In this study, we developed an integrated purificatio