A simple and robust reporter-based framework for deep functional characterization of PPARγ mutants

Missense mutations in nuclear receptors (NR) transcription factors (TF) cause a number of genetic disorders, including PPARG mutations that result in familial partial lipodystrophy type 3 (FPLD3). Experimental assessment is essential to establish a newly identified mutation as disease-causing, as accurately predicting the effect of new mutation in silico remains challenging due to the multifunctional and modular nature of these proteins. However, deep structure-function characterisation often re