Extracellular vesicles (EVs) represent an important mode of intercellular communication and cell regulation, by selective packaging and transfer of biological information between cells. The advent of quantitative mass spectrometry (MS)-based proteomics, lipidomics, and various other sequencing and profiling technologies, in conjunction with advances in molecular cell biology, imaging, EV purification strategies, single EV analysis, and informatics and data integration strategies [1], has contrib