Greenhouse gas nitrous oxide (N2O) emissions in soils are mainly driven by nitrification and denitrification, which are sensitive to environmental change. However, the response of soil N2O emission to N addition rates and elevated temperature in semi-arid grassland remains poorly understood, where nitrification dominates N2O emissions. We examined N2O emissions of a clay-loam loess to three N supply levels: no N supply (Control), 10 μg NH4+-N g1 soil per 3 days (continuous N supply, CN) and 100