From soil to crop: a tiered screening strategy for lipopeptide-producing Bacillus with antifungal, antibacterial and plant growth-promoting activities
Yuchao Ma
IntroductionTo address agrochemical pollution and improve microbial screening efficiency, this study aimed to develop an integrated pipeline for isolating high-yielding lipopeptide-producing Bacillus strains and validating their agricultural applications.MethodsA tiered screening strategy combining hemolytic activity assay, plate confrontation, and oil-spreading assay was established to isolate strains from rhizosphere soils. Lipopeptide production was identified by MALDI-TOF MS and quantified v