Computed tomography (CT) is an established imaging technique for diagnosing shoulder pathology in dogs. CT images can be used to measure muscle volume, however the reliability of measuring supraspinatus and infraspinatus muscle volume on CT images in dogs is unknown. The aims of this study were to assess the intra- and inter-rater reliability of muscle volume measurements of supraspinatus and infraspinatus in clinically asymptomatic dogs, evaluated on CT images. The CT images of 10 asymptomatic