Abstract Queueing migration is among the most intriguing forms of collective behaviour in Metazoa, yet its origin and evolution in terrestrial arthropods remain poorly understood because fossil evidence of collective movement is exceedingly rare and largely confined to Palaeozoic marine taxa. Here, we document an exceptional case of queueing migration in larval mites, Protofilum ordinatum gen. et sp. nov. (Trombidiformes: Erythraeidae), preserved in mid-Cretaceous Burmese amber (approx. 100 Ma).