A novel method for the histolocalization of Al and Pb in root tissues, tested in Typha domingensis Pers. grown in iron mining tailings

Abstract The objective of this work was to test a new method for Al and Pb histolocalization using Chrome Azurol S (CAS) and Pyrogallol Red (PGR), respectively, using roots from Typha domingensis Pers. plants grown in iron mining tailings. For both CAS and PGR, we tested two solvents (distilled water pH 6.7 and 100 mM phosphate buffer pH 5.0), two concentrations (0.1% and 0.5%, m V − 1 ), and three reaction times (1, 5, and 10 min). We tested CAS and PGR histolocalization of Al and Pb in T. domi