Abstract The rise of arboviruses in tropical countries, such as Brazil, underscores the need for new vector control strategies. This study examines the impact of indigo on the reproductive biology of Aedes aegypti. Females exposed to indigo during the larval stage (150 µg/mL) exhibited a significant decrease in fecundity (150 ± 41 eggs) compared to the control group (204 ± 42 eggs). Fertility was also affected, with lower egg viability and reduced metamorphosis at concentrations of 100 and 150 µ