KCa3.1 mediates radioresistance of silver nanoparticles in human glioblastoma cells
Francesco Ragonese·Bernard Fioretti·Luana Sallicandro·Alessandra Mirarchi·Gianmarco Reali·Antonella De Luca·Cataldo Arcuri·Sara Flamini·Oxana Bereshchenko·Paolo Cogliati·Roberto Spogli·Paola Sabbatini·Stefano Bruscoli·Paolo Sportoletti·Andrea Biagini
Abstract Silver nanoparticles (AgNPs) have emerged as a promising therapeutic tool for managing glioblastoma (GB), particularly in radiosensitisation. However, their mechanisms of action are not yet fully understood. Since ion channel activity is implicated in various aspects of radioresistance, we investigated the electrophysiological effects of AgNPs on GB cells. In the U251 human GB cell line, the acute application of AgNPs induced the activation of a cationic nonselective current in a dose-d