Green TLC-densitometric method for determination of cytarabine and granisetron in spiked human plasma: optimization and whiteness assessment

Abstract Cytarabine (CYT) is a cornerstone in the treatment of acute myeloid leukemia (AML) and is often co-administered with granisetron (GRS) to manage chemotherapy-induced nausea and vomiting (CINV). A precise and sensitive thin-layer chromatographic (TLC) method was developed and validated for the simultaneous determination of CYT and GRS in spiked human plasma samples. Chromatographic separation was achieved by using a developing system consisting of ethyl acetate, ethanol, and ammonia (3:6