A chromosome-level genome assembly of Vanilla planifolia uncovers the genomic architecture underlying partial endoreplication
Quentin Piet·Cyril Jourda·Carine Charron·Amaury Dedours·Michel Dron·Christophe Klopp·William Marande·Nathalie Rodde·Sonja Siljak-Yakovlev·Pascale Besse·Mickaël Bourge·Charlotte Cravero·Gaëtan Droc·Michel Grisoni·Fanny Lambert·Hadrien Máté de Gérando·Marine Sallaberry·Marilyne Summo·Stéphanie Bocs·Olivier Bouchez
Abstract Background Partial endoreplication is a prominent developmental feature and poses a significant challenge for whole genome assembly in orchids. This form of cell cycle results in highly unbalanced cell DNA content, with the highly endoreplicated (P) fraction being overrepresented in sequencing data compared to the non-endoreplicated (F) fraction. Results Here, we report the first genome assembly of Vanilla planifolia into 16 chromosome pairs using axillary buds enriched in non-endorepli