BSMV-mediated genome editing exhibits host-specific heritability: germline transmission in barley and somatic edits in Nicotiana benthamiana
Pankaj K. Bhowmik·Can Baysal·Brittany Polley·Naichong Chen·Naga Rajitha Kavuri·Wen Zang·Abdellah Barakate·Hui Yang·Murali Krishna Narra·Aaron D. Beattie·Colby Starker·Daniel F. Voytas·John T. Williams
Abstract Background Plant RNA virus–mediated guide RNA (gRNA) delivery represents a transformative advance in genome editing technologies. Unlike conventional transformation methods that rely on labor-intensive tissue culture and regeneration for each individual gRNA delivery, viral vectors can rapidly and systemically transmit gRNAs into pre-established Cas-expressing plants, providing an accelerated route for functional genomics and trait discovery directly in planta . However, key design para