Characterization of Fiber-Type Composition and Phosphoproteins of Fast- and Slow-Growing Broilers

Muscle fibers exhibit high plasticity: both the fast-twitch fiber type and slow-twitch fiber type can mutually transform under the regulation of phosphorylation. In this study, we characterized the muscle fiber profiles and phosphoproteomes of the m. extensor digitorum longus (EDL) and m. soleus (SOL) in slow-growing Xueshan chickens and fast-growing Ross 308 broilers. Fiber-type distribution was quantified by immunohistochemistry and RT-qPCR of MYH7B, MYH1A and MYH1B. TMT-based phosphoproteomic