Involvement of TRPA1 in Necrosis of Melanoma Cells via Phospholipase D1
Rei Nakano·Hiroshi Sugiya·Mana Sugimura·Naoya Yachiku·Nanako Kitanaka·Taku Kitanaka·Yoko Suwabe·Atsuto Naruke·Junichi Nunomura·Masami Uechi·Tomohiro Nakayama·Manami Kuji
The tumor microenvironment, including extracellular pH (pHe), has emerged as a key regulator of tumor cellular function. Although extracellular acidification sensing and function are well established, the effect of extracellular alkalinization on cellular functioning remains unclear. Here, we report that transient receptor potential ankyrin 1 (TRPA1) functions as an alkaline sensor and mediator of cell death in melanoma cells. Exposure to alkaline pHe (8.1) or allyl isothiocyanate (AITC), a TRPA