A Novel Peptide Derived from Sea Buckthorn Leaves: Enzymatic Preparation, Dual Inhibitory Activity Against α-Glucosidase and DPP-IV, and Its Molecular Mechanism

Sea buckthorn leaves are a relatively underutilised component of sea buckthorn processed products; however, various studies have indicated that they possess hypoglycaemic potential. Under alkaline solubilisation and acid-precipitation conditions, the extraction yield of sea buckthorn leaf protein (SLP) reached 19.33%. Trypsin was selected as the hydrolysing enzyme to extract SLPPs-T, with half-maximal inhibitory concentrations (IC50) against α-glucosidase and DPP-IV of 0.1361 ± 0.017 mg/mL and 0