Automated and scalable expansion of human liver organoids for translational applications

Human tissue-derived organoids hold strong potential for personalized medicine and cell therapy, but this requires large cell quantities. Conventional organoid culture systems remain labor-intensive, are difficult to scale, and lack process control. Here, we present a novel strategy using an automated bioreactor platform that enables large-scale expansion of human liver organoids. Human liver organoids were expanded for 14 days in a single bioreactor suspension culture bag and compared with spin