Monoclonal antibodies against nitrated nerve growth factor reveal an oxidation-dependent pathogenic hallmark in ALS
Valentina Varela·Luis Barbeito·Emiliano Trías·Ari Zeida·Monique Richter·Andrés de León·Ernesto Miquel·Peter H. King·Brigitte Vulliez-Le Normand·Mariano Martinez·Pedro M. Alzari·Silvina Bartesaghi·Rafael Radí·Santiago Garcimartín
Nerve growth factor (NGF) is a member of the neurotrophin family, essential for neuronal survival and phenotypic maintenance. However, in vitro, its function can be disrupted by oxidative posttranslational modifications such as tyrosine nitration. Nitrated NGF (NO 2 NGF) has been shown to have a gain-of-toxic, pro-apoptotic, activity in motoneuron cultures. Herein, we report the generation and characterization of monoclonal antibodies (mAbs) that specifically recognize NO 2 NGF to unravel its fo