Although renal disease in Syrian hamsters (SHs) has been reported, imaging studies of normal kidneys in this commonly used pet and laboratory species are lacking, despite the key role of imaging in diagnosis. This study aimed to examine kidneys of Syrian hamsters using radiographic and anatomical methods, focusing on kidney location, visibility, size, and its ratio to the second lumbar vertebra, along with the effects of sex, body size, side, and recumbency. Abdominal radiographs were obtained f